SCI 250 Week 1 Identifying Bacteria

SCI 250 Week 1 Identifying Bacteria

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SCI 250 Week 1 Identifying Bacteria

Review Figure 6.25 and Table 9.5 of Microbiology.

Complete Appendix B to classify bacteria and identify a strain of bacteria responsible for a simulated food poisoning outbreak.

Appendix B – Identifying Bacteria

Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests. The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. When a microbiologist needs to identify a specific bacterium, biochemical tests are used.

PART ONE: GENERAL BACTERIA IDENTIFICATION

Review the dichotomous key in Figure A, the bacterial shapes in Figure B, and the Gram stain information below. You will use all three to determine to which major group unknown bacteria belong.

Gram Stain Results:

Purple = Gram positive

Red = Gram negative

Neither purple nor red = No cell wall (neither Gram positive nor Gram negative)